home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9408e.zip
/
M9480831.TXT
< prev
next >
Wrap
Text File
|
1994-09-05
|
3KB
|
46 lines
Document 0831
DOCN M9480831
TI Genetic variation within and between human, rat and mouse-derived
Pneumocystis carinii.
DT 9410
AU Keely SP; Pai HJ; Sunkin SM; Baughman R; Sidman CL; Stringer SL;
Stringer JR; University of Cincinnati College of Medicine, Ohio.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:590 (abstract no. F-10). Unique
Identifier : AIDSLINE ASM94/94313114
AB Pneumocystis carinii is an opportunistic pulmonary pathogen that causes
P. carinii pneumonia (PCP) in immunocompromised individuals and is the
major cause of death in AIDS patients. The organism thrives in a wide
variety of mammals and is generally referred to as P. carinii regardless
of host species. Recent reports indicate that genetic differences exist
between organisms isolated from humans, ferrets and rats and that
genetically distinct organisms can propagate within a single host. In
order to further examine this diversity within and between host species
we have sequenced a portion of the thymidylate synthase (TS) and
mitochondrial large subunit rRNA (mrRNA) genes of P. carinii. P. carinii
TS and mrRNA genes were sequenced and compared from organisms derived
from scid mice, immuno-suppressed rats and AIDS patients. Novel P.
carinii TS and mrRNA sequences were attained from each of these hosts.
Nucleotide variation of these two genes within a single host was
detected in humans and rats, but not in mice. Specifically, TS and mrRNA
sequences showed deletions and point mutations in P. carinii isolated
from rats. Sequence at the 3' end of numerous human P. carinii mrRNA
genes revealed nucleotide base substitutions. Human P. carinii TS and
mrRNA sequences were shown to be equally divergent from homologous genes
of P. carinii rat and mouse-derived organisms. Surprisingly, nucleotide
alignments of the P. carinii TS and mrRNA genes show that P. carinii
from rats are as equally divergent from each other as they are from
mouse P. carinii. Taken together, these data show that humans, rats and
mice harbor distinct forms of P. carinii, that nucleotide polymorphisms
occur within a single host, and that organisms isolated from these three
mammalian hosts are probably different species of P. carinii.
DE Acquired Immunodeficiency Syndrome/*COMPLICATIONS Animal Base Sequence
Comparative Study *Genes, Fungal Human Mice Mice, SCID Pneumocystis
carinii/*GENETICS/ISOLATION & PURIF Pneumocystis carinii
Infections/ETIOLOGY/*MICROBIOLOGY Point Mutation Rats RNA,
Fungal/GENETICS Sequence Deletion Thymidylate Synthetase/GENETICS
*Variation (Genetics) MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).